Results
| PMID | 8612850 |
| Gene Name | TNF |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 25 |
| Population details | 25 (17 women with endometriosis, 8 fertile controls undergoing tubal ligation) |
| Sex | Female |
| Associated genes | TNF alpha, IL-8, IL-10 |
| Other associated phenotypes |
Endometriosis |
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Fertil Steril. 1996 May;65(5):925-30. Rana, N| Braun, D P| House, R| Gebel, H| Rotman, C| Dmowski, W P Institute for the Study and Treatment of Endometriosis, Grant Hospital, Chicago, Illinois 60614, USA. OBJECTIVE: To evaluate basal (constitutive) and stimulated synthesis of tumor necrosis factor alpha (TNF alpha), interleukin (IL)-8, IL-10 by peritoneal macrophages (PM) in women with endometriosis. DESIGN: Peritoneal macrophages were cultured in the presence or absence of lipopolysaccharide (LPS) for 24 hours. Peritoneal fluids (PF) and PM supernatants were assayed for cytokines using ELISA. SETTING: Institute for the Study and Treatment of Endometriosis and university-based research laboratories. SUBJECTS: Fertile controls undergoing tubal ligation (n = 8) and women with endometriosis (n = 17). INTERVENTION: Peritoneal fluid samples were obtained at the time of diagnostic laparoscopy (endometriosis group) or laparoscopy for tubal ligation; both were performed in the midluteal phase of the cycle. RESULTS: Both basal and LPS stimulated production of TNF alpha, IL-8, and IL-10 by the PM were elevated significantly in women with endometriosis as compared with the fertile controls. CONCLUSIONS: This study demonstrated that cytokines TNF alpha, IL-8, and IL-10 are synthesized at greater than normal levels by basal and stimulated PM from women with endometriosis. The levels of TNF alpha and IL-8 correlated with the levels in the PF, suggesting that PM are the principal source of these cytokines in the PF. Mesh Terms: Ascitic Fluid/metabolism| Cells, Cultured| Cytokines/*biosynthesis| Endometriosis/*metabolism| Female| Humans| Interleukin-10/biosynthesis| Interleukin-8/biosynthesis| Lipopolysaccharides/*pharmacology| Macrophages, Peritoneal/*metabolism| Tumor |
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